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The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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A schematic diagram with the primary structure of the glycan is shown in Fig
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Henson), National Jewish Medical and Research Center, Denver, CO
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The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques
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The error in the R^sub 1^ and R^sub 2^ values was defined as the standard deviation between three curve fittings, one with all time points and two fittings with reduced data sels containing every second time point, which were shifted either zero or one time point in the relaxation series
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1 C was rapidly cooled to 5°C and scanned again from 5 to 100°C
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The herein-discussed polymorphism of insulin amyloids, and the putative lateral assembling interactions between the seedlike forms and early nativelike molecules, are important-since both appear to correspond to in vivo cases of amyloid proliferation, and may contribute to a deeper understanding of the amyloidal-type of neurotoxicity, as well as the intra- and extracellular mechanisms of the propagation of amyloids
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