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Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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6), although isothermal melt done with rSBA at this protein concentration still shows a three-state unfolding profile
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Inasmuch as sequence-specific effects are present over five- to nine-residue stretches, it is conceivable that there are native-like local biases as well as default biases for conformations such as polyproline II (48)
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A review of linear chain properties
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Preliminary Fourier transform infrared spectroscopy (FTlR) and circular dichroism (CD) thermal denaturations at physiological pH on the EI enzyme of S
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It is obvious from the data shown in Fig
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Molecular modeling of the amphipathic helices of the plasma apolipoproteins
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. How the dilatation symmetry of denatured states is broken under folding conditions will depend on a variety of factors including local biases for turns and short stretches of extended or helical conformations, the drive to sequester hydrophohic amino acids, and the achievement of specificity in side-chain packing (103)
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. We interpret these results such that although diminazene penetrates the cell membrane earlier than aceturate due to higher permeability, a large portion of it is strongly bound, and only a small portion of diminazene molecules weakly bound to nuclear macromolecules gives the initial signal
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