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The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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These results suggested that during ACYI thermal denaturation, the dominant events were the unfolding of the β-sheet (1638 cm^sup -1^) and the formation of the intermolecular β-sheet in aggregates (1617 cm^sup -1^)
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Anilinonaphtalene sulfonate as a probe of membrane composition and function
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DISCUSSION Subcellular spectroscopy The negligible lipid signal in the spectrum obtained from the voxel in the nucleus demonstrates that although localization errors are not entirely evitable in high-field MRS, there is no significant signal spillover from the surrounding lipid-rich cytoplasm
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Several amyloidogenic in vitro assays employ acidic pH for generation of amyloid fibrils; however, ThT shows very modest fluorescence at acidic pH when bound to protofibrillar amyloid of TTR (Fig
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These results, and those using CPM-PLCβ gave similar volume changes which were approximately twice as high as that seen for the associations on membrane surfaces
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In the final stages of vitellogenesis, the largest oocytes were approximately 700 [mu] in diameter and had numerous oil vacuoles and yolk globules throughout the cytoplasm
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Temporal patterns in parturitionFrom 1996 through 1998 we estimated relative larval production for four age groups: 6-8; 9-11; 12-14; and 15 years and older (Fig
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The samples were treated in papain digest buffer, as described above
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