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. This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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. The energies were calculated every 5 ps as reported earlier by Hsu and Bonvin, 2004, and therefore N is 16,001 when analyzing the 2-10 ns simulation segments
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. The IR and fluorescence results strongly suggested that the thermal unfolding of ACYI could be illustrated as a two-stage process involving an intermediate: native state (N) [arrow right] intermediate stale (I) [arrow right] aggregates (A)
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. Accurate models for denatured states also impact a range of areas, including quantitative studies of in vitro folding pathways (10,19-24), protein design (25,26), studies of protein aggregation (27-29), and understanding preferential interactions in cosolute mixtures (1,3,30-36)
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. The distributions of distances are sharply peaked for near-neighbor residues and they become increasingly broad as sequence separation increases
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.2-cm cell at a protein concentration of 0
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. Furthermore, the cooling curve of the apoA-2:DMPC sample shows partial protein refolding below 80°C, followed by additional refolding between 32 and 24°C, i
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.5 nm observed after sample exposure to high temperatures (lanes 5 and 6 in Fig
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