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. In the case of azurin, to be consistent with the previous study (12), the protein concentration was raised to 2
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. Arrhenius plots for these phases are linear and intercept near 100°C (Fig
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. This leads us to further understanding of intermediate protein structures in the unfolding process
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. For the longest sequence in our data set-the sequence of villin-for which N = 126, generation of the desired conformational ensemble takes ~ 20 h on a single 2
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. It is evident that a larger number of IMCs in the bulk of the solder were found for 275°C (Fig
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. The neighboring residue Q40, which lies at the center of the complex interface, shows the largest reduction among all residues (top row in Fig
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