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. For example, the HIV 1 gp-120 protein fails to reach its final folded stale in the absence of glycosylation, whereas the nonglycosylated RNase A has lower folding rates compared to its glycosylated counterpart, RNasc B (10-12)
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. The accuracy of these ensembles is judged by matching the structural characteristics of the simulated ensembles to those predicted by field theories
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. and from Aspergiltus oiyzae (TAKA; from Sigma) was obtained as lyophilized powder
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. This is far from the lower micrometer range typical of cellular dimensions
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. Water and lipid MRI of the Xenopus oocyte
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. © 2005 by the Biophysical Society0006-3495/05/01/15/10 $2
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. The efficiency E of this process exhibits a strong distance dependence, given by E = 1/(1 + (R/R^sub 0^)^sup 6^), where R is the donor-acceptor distance and R^sub 0^ is the Förster radius at which the efficiency is 50%
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. The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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. This modeled structure has allowed us to explain the experimental conformational and thennodynamic features of the protein
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. Finally, we studied the conformational and stability properties of EI^sup sc^ in the presence of the effectors (i
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. Water and lipid MRI of the Xenopus oocyte
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