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5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig
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Glycosylation:heterogeneity and 3D structure of proteins
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The goodness of solvent can be assessed by comparing the measured scaling of chain size with chain length (N) to that obtained by assuming the EV limit
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The measurement was repeated on five individual oocytes
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We conclude that HuBChE stabilization by covalent modification with soman is due to an increase in the free energy of the unfolded state
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The data are presented following the flow diagram of the PINATA script
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If a protein packs well with the membrane interface, then pressure is not expected to destabilize its membrane interactions
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Structure, function, and control of phosphoinositide-specific phospholipase C
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Cross-linking studies of the self-association properties of apo-A-I and apo-A-II from human high density lipoprotein
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33 w/d20OB-14/95 Presence 13
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4), the entropy of most of the structural elements involved in the gp120/CD4 interface starts to level off after 4 ns as a result of their smaller number of atoms
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SpeciesSampling location eggsday 1Kansai Environmental Early 20 Apr '95Engineering Center(Miyazu, Kyoto Pref) 2Kansai Environmental Late21 Apr '95Engineering Center(Miyazu, Kyoto Pref
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There is reason to believe that the [[epsilon]
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t] = 1] - E[[DaysNSDPop
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"We deeply regret having to take such serious actions, but feel that it needs to be made clear to candidates that NABP does not tolerate such security breaches," said NABP president John Fiacco
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."The people PowerSwipe helps most are those with higher transaction tickets and higher transaction volumes," says Elias
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. Molecular modeling of the SBA glycoprotein In the Protein Data Bank, none of the representative structures of soybean agglutinin show the entire covalently linked glycans
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. In this case, deglycosylation lowers the T^sub m^ of the peptide by 20°C, whereas the free energy of unfolding is reduced by only 1 kcal/mol (26)
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. Immunol Cell Biol 2003; 81:289-296(51) Kirkham PA, Spooner G, Rahman I, et al
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. Protein concentration was determined from the absorption at 280 nm and enzymatic activity was tested before and after neutron scattering experiments (36) at 25°C in a 25-mM phosphate buffer (pH 7
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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. ThT, on the other hand, requires a pH adjustment for its characteristic amyloid fluorescence signal (Fig
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. The consequent mutual but general attraction of the helices for each other permits the final diffusive search for the native state to take place in a two-dimensional space, rather than the full five-dimensional search configuration space of all the diffusive degrees of freedom available to the protein once the secondary structure has formed
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. At stage 2, parturition would take place in 37 days--stage-1 duration (~2 days) = 35 days
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.4 NA oil immersion objective (Zeiss)
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. Stability of hairpin ribozyme tertiary structure is governed by the interdomain junction
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