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The amide I' bands were assigned according to previous publications (24)
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REFERENCES L Rudd, P
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10 for three proteins of different lengths
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0, when the protein carries a net negative charge, can be described by hard sphere behavior those at pH 3
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. This led us to shift the excitation to a lower wavelength (270 nm) to minimize the contribution from Rayleigh scattering in the emission spectra
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