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Samples were frozen in liquid nitrogen and stored at -80°C
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Decomposition of protein tryptophan fluorescence spectra into log-normal components
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Recently, a study conducted in our laboratory investigated the possibility to unveil potential alterations of the protein tertiary structure in ice from binding of ANS (12)
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5°C) with respect to its native counterpart
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Two invertebrate acetylcholinesterases show activation followed by inhibition with substrate concentration
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6, whereas Table 1 collects the data for residues with significant exchange broadening (see table legend for more details)
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Linear viscoelasticity of colloidal hard-sphere suspensions near the glass-transition
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Bis-ANS shows a wide variety of fluorescence responses and fluoresces most strongly when bound to monomeric A-state TTR and surprisingly also fluoresces strongly when bound to unfolded monomeric TTR
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The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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The pleckstrin homology domain of phospholipase C-β^sub 2^ links the binding of Gβγ to the catalytic core
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The conformational entropy change of gp120/CD4 complexation roughly agrees with the experimental value despite the fact that the contribution from bulk solvent was neglected in our analysis
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Particle formation depends on various solution parameters and cooling conditions
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Isopar G, H, and L contain hydrocarbons with 10-11, 11-12, and 11-13 carbon atoms, respectively, and were used as received
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They were fixed with 5% formaldehyde in sea water, either immediately or after incubation in seawater in finger bowls at 20[degrees]C for 24 hours, and identified by careful observation as described by Ikeda and Mito (1988) and Ikeda et al
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Our results indicate that an early step in the 4WJ ribozyme folding pathway likely involves the formation of a fast equilibrium between extended undocked and quasi-docked structures (Fig
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20Preparation and maintenance of pellet cultures
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, director of the National Human Genome Center at Howard University in Washington, D
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004) for the overall sample (r = -0
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