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The spectra recorded at temperatures from 68°C to 72°C were not included in the 2D IR analysis in Fig
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To assay conformational preferences of highly denatured proteins, we quantify a variety of properties for EV-limit ensembles of 23 two-state proteins
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Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution
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To further demonstrate the use of fluorescence anisotropy decay, we probed TTR aggregation kinetics using time-resolved anisotropy
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The increased temperature shortens the lag phase of the aggregation, which is defined as lapse-time passed until the appearance of first fibrillar forms (rather than of transient early amorphous aggregates-see Fig
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t] as described by (2) and (3) above
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. Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure
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. Here, [left angle bracket]R^sup 2^^sub e^[right angle bracket] is the mean-squared end-to-end distance, R^sup 2^^sub ij^ = |r^sub i^ - r^sub j^|^sup 2^, and we choose r^sub i^ and r^sub j^ to be the position vectors of α-carbon atoms of residues i and j, respectively
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. ThT does not show binding to the U-state and only shows a minor burst phase, indicating that this is a clearcut aggregation probe
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