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and hence this wavelength was chosen as the emission wavelength for the isothermal denaturation melts
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However, on increasing the denaturant concentration further, to 3
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The tertiary structure thus obtained was covalently linked to the Nδ52 of Asn-75 of each of the subunits in the protein
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The interactions between the attached glycans and the protein were assessed using the online LPC server (24)
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Direct characterization of the folded, unfolded, and urea-denatured states of the C-terminal domain of the ribosomal protein L9
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Even under these conditions, the theoretical displacement error is 27
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5 min, exponential filter width = 20 Hz, temperature = 18°C
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1 is used) assuming the apparent specific volume of the protein phase to be 1
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1 C) is invariant in repetitive CD and DSC scans (curves 2-4, Fig
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