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. Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure
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. Crystal structure analysis of oxidized Pseudomonas aeruginosa azurin at pH 5
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. In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes
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. To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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. On average, these fibrils are -80-500 nm long, and rather uniformly 4
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. We determined stage-specific fecundity in black rockfish for females with unfertilized yolked oocytes (n=184) and fertilized eggs (n=85)
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. The ribozyme system is sampled at 40 times per measurement interval to simulate the effects of the threshold of 40 photons we used for the FRET analysis
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. Resin blocks were further trimmed after which 90 nm sections for TEM were cut with a LKB Ultratome III ultramicrotome using a diamond knife
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. Data AnalysesDescriptive statistics were used to characterize the sample
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. These folding intermediates exhibited flexible tertiary structures and nativelike secondary structures
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. The inability of two- and three-way junction ribozymes to fully populate a docked conformation reveals the importance of correct helical junction geometry as well as loop elements for effective ribozyme folding
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