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Two-dimensional infrared correlation (2D IR) analysis was carried out using SDIAPP software developed in-house (27) according to the generalized two-dimensional (2D) correlation algorithm (29)
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Excitation wavelength was 370 nm, and the emission intensity was collected from 430 to 700 nm
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On the other hand, at acidic pH, EI^sup sc^ lost its tertiary structure and solvent-exposed hydrophobic patches, as suggested by 1), the changes in the fluorescence intensity (Fig
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The measurement was repeated on five individual oocytes
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1 A shows the ^sup 1^H MR image of a single cell, a stage VI X
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Establishing quantitative relationships between thermal fluctuations and functional motions is challenging, taking into consideration the vast variety of protein functions (synthesis, catalysis, electron- and proton transfer, etc
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Li+ shows moderate chemical potential in PEG 3000 but is the weakest DNA compaction agent in PEG 10000
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Thus, pressure methods allow for the estimation in the volume associated with the Gα^sub s^(GDP) deactivation of the PLCβ-Gβγ complex even though the change in interaction energy between PLCb and Gβγ caused by Gα^sub s^(GDP) could not be detected by spectroscopic atmospheric pressure measurements
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Instead, it exploits the lower energy state of the entire (d - n - 1)^sup th^ dimensional subspace to reach it via a one-dimensional diffusion in the (d - n)^sup th^ dimension, which it performs first
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In the hypergutter picture, the nine-dimensional search space of these critical regions separately is reduced to three sequential three-dimensional searches by the long lifetimes of the native regions when the remainder of the protein is disordered
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Another recent, theoretical, contribution has pointed out that fine-structure of a few k^sub B^T within the transition state on a reaction pathway can accelerate folding (Wagner and Kiefhaber, 1999)
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The covariance matrix and thus the configurational entropy were calculated for three different sets of atoms (see Table 1):1
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