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. © 2007 by the Biophysical Society 0006-3495/07/01 /208/09 $2
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. These maps show regions where contacts are either present (strong) or absent (weak) in both the native and denatured states
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. Unlike simple hyperbolic binding curves, profiles such as those of Fig
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. The individual response to freezing differs considerably, on the other hand, with regard to the number of binding sites n increasing two and three times relative to C3A/C26A in I7S and F110S, respectively
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. Circular dichroism spectroscopy and light scatteringCircular dichroism (CD) and light-scattering data were recorded using AVIV-215 spectrometer (AVIV Biomedical, Lakewood, NJ) with fluorescence accessory and Pelletier temperature control
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. 4 B at 8 ns), regardless of the order of appending
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. Concomitantly, the third hypervariable loop (V3) undergoes a rearrangement to a somewhat different conformation leading to accessibility of the epitope for co-receptor binding
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. The interaction was not significant, F (1,140) =
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