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The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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These observations in the IR spectra coincided with the deduction based on DSC results that the thermal unfolding of ACYI is an irreversible process accompanied with protein aggregation
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Even after increasing the metal ion concentration to as high as 30 mM, the refolding yields remain unchanged
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Address reprints requests to José L
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Although the three-dimensional structure of these α-amylases show up rather similar, the corresponding stabilities differ significantly (16,17)
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Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
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Sample preparation Before sample preparation, all protein stocks were dialyzed against 2 mM Hepes buffer, pH 7
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Brochier,[double dagger] B
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How has such a remarkable reduction in folding time been achieved without the use of a spatially "funneled" energy landscape? Of course energetic interactions have been implied, but these have not been of the spatially-extended type
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In detail the folding energy landscape will look more like a series of low-dimensional terraces (inset to Fig
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W/d=missing data
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The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements
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