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Thus the intrinsic Trp fluorescence was used to investigate the changes of the tertiary structure of ACYI during thermal denaturation
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For experiments involving PEP, control scans were acquired with PEP added to buffer versus buffer in the reference cell, and baselines were found to be the same as in buffer-buffer baselines, suggesting that under these conditions hydrolysis of PEP during heating was not significant
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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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The discrepancy at the lower concentrations (mass fraction ~7-16% w/w) could be decreased if the assumed molecular weight (MW) were reduced from the dimer MW to the monomer MW
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The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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[FIGURE 6 OMITTED]DiscussionLiterature describing the early stages of species belonging to the family Pinguipedidae (formerly Mugiloididae) is scarce
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t] refers to the total number of days NSAID therapy dispensed in month t
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) Moreover, our data exclude patient spending on nonreimbursed over-the-counter analgesics, such as low strength ASA
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