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In all fluorescence experiments the samples were prepared from ANS and protein stocks to a final constant protein concentration of 2 µM in 2 mM Hepes buffer, pH 7
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Direct determination of vibrational density of states change on ligand binding to a protein
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Given the anisotropy and the maximum (τ^sup o^^sub m^), τ^sup o^^sub 1^, the angle Φ that each N-H^sup N^ bond vector makes with the main axis of the diffusion tensor can be calculated
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Briefly, we find: i), the observed 2
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In practice, the large difference in anisotropy decay between monomeric forms lead to a broad baseline with the contribution of the smaller monomeric forms contributing as a decay curve superimposed onto a baseline
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5 A, and see below)
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How has such a remarkable reduction in folding time been achieved without the use of a spatially "funneled" energy landscape? Of course energetic interactions have been implied, but these have not been of the spatially-extended type
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By 9-10 mm BL, dorsal (V+26-27) and anal (II+20-22) fin elements reached their full complement
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Descripcion de tres larvas de peces teleosteos del Mar Argentino: Mugiloididae, Ophidiidae (Genypterus blacodes) y Tripterygidae (Tripterygion cunninghami)
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This issue and appropriate control experiments are discussed further in the Results section
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