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Each straight line in the phase diagram reflects an "all-or-none" process, and the joint position of two lines for a transition process indicates that an intermediate appeared at the corresponding temperature
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two peaks with different thermodynamical properties are observed, one corresponding to EIN (with pH-independent T^sub m^, values) and a second corresponding to EIC (with pH-dependent T^sub m^-values) (6)
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This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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Transient clustering in a protein solution
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Chemical cross-linkingA total of 100 µl of aliquots from a TTR misfolding reaction was withdrawn al different time points, and 100 mM phosphate buffer was added in addition to 10 µl of glutaraldehyde (25%, electron microscopy grade (Sigma, St
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1 B, solid circles) mainly reflects the formation of the protein-containing vesicles rather than intact-size disks
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