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In line with these results, the presence of MG intermediate states in the unfolding of S
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Thermal denaturation measurements Thermal unfolding curves were determined in the presence of 1 mM β-mercaptoethanol by following the emission fluorescence at 315, 335
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The overexpressed dimeric enzyme has four glycosylation sites per monomer, with carbohydrates accounting for ~10% of the dry weight
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6c and/or 5), establish whether fast exchange applies or not without prior knowledge of the exchange timescale
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To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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Aliquots of the aggregation reaction were withdrawn and mixed with an assay buffer (10 mM HCl, containing a final concentration of ANS, Bis-ANS, or DCVJ at 2
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1, A and B; curve 1, Fig
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Nevertheless, this new paradigm will apply to all foods
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Johnson Space Center, Houston, Texas
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Since we use relative peak positions and distance (inter-arm proximity) changes in our discussion here (and not absolute distances), variations of [gamma] from 1 do not affect the discussion or conclusions of this study
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A 100 µl aliquot of both the samples and standards was pipetted into a 96 well plate
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