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The backbone (N, Cα, and C') atoms of individual residues were used for superposition of trajectory configurations when calculating the entropy per residue (code: fir)
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6 shows the derivative plot of the respective thermal melts
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Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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A rough estimate of the minimum number of binding sites, as done above with azurin, yields n ~20, which is four times larger than the corresponding value in liquid solution (5)
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