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Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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Kinetic resolution of unnatural and rarely occurring amino acids: enantioselective hydrolysis of N-acyl amino acids catalyzed by acylase I
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(21) This, however, does not preclude the possibility that some receptors may ultimately be shown to perform both tethering and tickling (signal transduction) functions
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The distortion of the native structure inferred from ANS binding was found to draw a parallel with the extent of irreversible denaturation by freeze-thawing, suggesting that these altered conformations play a direct role on freeze damage
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The decreased strength of hydrophobic interactions at low temperature and the peculiarly high solute concentration (~5 M) and ionic strength of the liquid water pool may be factors that contribute to the lower affinity of ANS for proteins in ice
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1 B, solid circles) mainly reflects the formation of the protein-containing vesicles rather than intact-size disks
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The diffusion-collision model and experimental data
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The triboelectric numbers of the developer (electrostatic charge per mass of the developer, µCoulombs/g) were measured with an apparatus [14] based on the blow-off method
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