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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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The phase diagram analysis, which is a sensitive tool to detect folding intermediates (35), was performed to characterize whether an intermediate was involved in ACYI thermal unfolding
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This helix-loop-helix motif folded against the ban-el, covering a hydrophobic patch (which includes Tip^sup 461^): the motif was linked to the barrel by two salt bridges, namely, Arg^sup 537^-Asp^sup 467^ and Arg^sup 546^-Asp^sup 459^ (Fig
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In contrast PPA shows already in the native state a rather red-shifted emission spectrum (λ^sub max^ = 343 nm) which first decreases in intensity upon heating
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Optical spectroscopy to characterize protein conformation and conformational changes
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, 1999) have also been demonstrated
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Additionally, the quasi-docked species appears to form very rapidly (see below), suggesting that this species could act as a kinetic intermediate in the docking pathway of the natural ribozyme
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A significant Advice x Block interaction indicated that this trend differed somewhat between the two advice groups, F(7, 98) = 2
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