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Besides, complex formation between GR and NADPH was only detected in the dimeric form of the enzyme, which would argue that NADPH does not participate directly in the process of GR folding/assembly
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Further, the unfolding pathways in the two are widely different
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The λ^sub max^-values of the unfolded states in NDSB solutions were slightly larger (see Fig
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Further, their internal flexibility increases drastically from compact WT and C3A/C26A to the cavity-forming mutants I7S and F110S
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This is in contrast with stabilization by kosmotropic buffers, which decrease the free energy of the native state
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As can be seen, the RT^sup n^^sub 1^ values spread around RT^sup n^^sub 1^ [asymptotically =] 0
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, 1976); however, the detailed characterization of DNA secondary structure of Ψ-condensates has not been yet performed
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A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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16 M KCl, 1 mM EGTA, pH 7
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. Moreover, resolution-enhancing techniques, such as second derivative and 2D IR, have been shown to be sensitive tools to monitor the sequential events in protein conformational changes by FTIR (27,28,30,38-42)
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. In the case of the SL 4WJ, for the broad/asymmetric peaks at some of the high Mg^sup 2+^ concentrations, these fitted Epeak values are representative of the peak positions, but are higher than the average FRET efficiencies by ~0
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