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Denaturing behavior of glutathione reductase from cyanobacterium Spirulina maxima in guanidine hydrochloride
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Parameter A, which is a sensitive tool to reflect the position and shape of the Trp fluorescence spectrum, slightly decreased when the temperature increased to 64°C, and then an abrupt decrease was observed
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and acquired every 3 K
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(58)PPAR[gamma], agonists enhance efferocytosis by human alveolar macrophages through the increased expression of CD36
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The photomultiplier (EMI 9635QB) current was amplified by a low-noise current preamplifier (Model SR570, Stanford, Sunnyvale, CA) followed by a lockin amplifier (Model 393, ITHACO, Ithaca, NY) operating at the chopper frequency
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Here we report a study of the backbone dynamics by ^sup 15^N-spin relaxation on the CaM dimer in complex with a dimeric peptide that mimics the CaM binding region of the bHLH transcription factor SEF2-1
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Both the analytical expressions for tumbling times of cylinder symmetric objects as well as bead model hydrodynamics incorporated in the DASHA software (24) were used (see Supplementary Materials for more details)
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Thus, the data are consistent with a motional model in which the two N-terminal and two C-terminal CaM domains in the dimeric CaM:SEF2-lmp complex undergo a small-scale wobbling motion with a half-angle of ~20° as estimated from S^sup 2^^sub s^ = 0
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The rationale of this work was to inquire into the hierarchical assembly of the insulin amyloid and link the morphological transitions to conformational events observed by other biophysical and spcctroscopic methods
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De Cabo (3) reported pinguipedid larvae from the Argentine shelf but did not identify the specimens to species level
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Supernatant solutions were decanted, and carbonate precipitates were washed free of soluble salts by repeated decantations with deionized water
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The federal government should supplement what the private sector can do
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