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. Circular dichroismFar-UV CD spectra of GR were measured on a JASCO (Tokyo, Japan) J-715 spectropolarimeter (kindly made available by Dr
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. Second, unlike hard-sphere potentials, which stipulate that all sterically allowed conformations are isoenergetic, soft-core potentials encode the requisite conformational specificity (48,53,58)
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. Specifically, we ignored the interactions between atoms in residues whose C^sub α^-C^sub α^ distance exceeds 15 Å because the inverse power potential energy for these distances is nearly zero
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. FTIR The protein was speed-vacuum dried (Speed-vac rotatory evaporator, Savant Instruments, Farmingdale, NY) and dissolved in the deuterated buffer containing 10OmM NaCI
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. It was pointed out that this trend probably reflects multiple binding sites of varying affinity, likely a consequence of the broad distribution of protein conformations in ice (3)
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. In an attempt to correlate affinities and n values to specific aspects of the macromolecule, we observe that all mutants are less stable than the WT by a comparable amount, the denaturation-free energy change, ΔG° at 20°C, decreasing from 6-7 to ~3 kcal/mole (20,27)
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. Weisblat (United States) for suggestions and comments in its revision
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. The cross sectioned samples were then finally gold coated and prepared for analysis
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. Diseases of protein conformation: what do in vitro experiments tell us about in vivo diseases? Trends Biochem
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. This function accounts, in part, for the anti-atherogenic action of HDL and its major protein, apolipoprotein A-1 (apoA-1, 243 aa) (Barter and Rye, 1996)
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. Comparison with apoA-1 and apoC-1To assess the relative stability of the DMPC complexes with various human apolipoproteins, we used DSC, CD, and light scattering to monitor heat denaturation of similar-size DMPC disks with apoA-1, apoA-2, or apoC-1 under otherwise identical conditions
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. This corresponds to the existence of narrow gullies in the hypersurface that are hard to find
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. Most of the methanol was decanted, and the remaining methanol was allowed to evaporate
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. There was no problem obtaining a large amount of the monoclonal antibody required when identifying P
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. Methods and MaterialsAssessment of cell number and viability
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. We need to teach people how to stock up, how to use us to replenish their supply of toothpaste and shampoo and other basic OTC products
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. While we do not have data on patient health outcomes and individual patients commonly report better efficacy and/or tolerability with particular NSAIDs (Walker, Chan, and Yood 1992; Langman et al
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. Data AnalysesDescriptive statistics were used to characterize the sample
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. MethodParticipantsThe participants were 16 undergraduate students at The University of Hong Kong
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