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Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding center department direct education loan servicing us .
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Since PLCβ and G-protein subunits associate on membrane surfaces, we worked under conditions where the membrane surface properties are not expected to change center department direct education loan servicing us .
The model described above implies that activation of PLCβ2 by Gβγ-subunits occurs through one or more small interactions whose energies are within the experimental error of the measured affinities center department direct education loan servicing us .
It is notable that although pressure techniques have been used to monitor the associations between aqueous soluble proteins, pressure has not yet been applied to monitor lateral associations of membrane-bound proteins (for recent reviews, see Ernst, 2002) center department direct education loan servicing us .
As an example of the type of fine-structure predicted, the model contains a natural explanation of the curvature seen in plots of the denaturant dependence of folding rates center department direct education loan servicing us .
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