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Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione ReductaseLouzada, Paulo RobertoABSTRACTThe equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated
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The reason that the autopeak at 1629 cm^sup -1^ did not appear might have been due to the overlapping of the adjacent intense peak at 1638 cm^sup -1^
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Inspection of Kratky profiles in these two regimes suggests the following: in the EV limit, there are two discernible length scales
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It can be concluded, then, that EI^sup sc^ at low pH unfolded via a moltenglobule-like species, as it has been described in other large multimeric proteins (49)
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Plenum Press, New York
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4 A), up to sixfold, whereas the onset of binding occurs at either smaller or higher ANS concentrations, indicating larger and smaller binding affinities, respectively
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Above that temperature, the MSDs for HuBChE-D^sub 2^O are larger than those for Soman/HuBChE-D^sub 2^O, which remain in a quasiharmonic regime, as shown by their linear dependence on temperature (Fig
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Gabel's present address is European Molecular Biology Laboratory, Heidelberg, Germany
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Thus, the presence of nanosecond-timescale internal motion is directly evident from the observation that RT^sup n^^sub 1^ is smaller than a critical value determined by the experimental error in the R^sub 1^ measurements
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Two ml of a buffered BLG solution was dialyzed against buffered PEG solutions of known osmotic pressure (15,16) at 20°C for 24 h
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16 M KCl, 1 mM EGTA, pH 7
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Kinetic Stabilization and Fusion of Apolipoprotein A-2:DMPC Disks: Comparison with apoA-1 and apoC-1Jayaraman, ShobiniABSTRACTDenaturation studies of high-density lipoproteins (HDL) containing human apolipoprotein A-2 (apoA-2) and dimyristoyl phosphatidylcholine indicate kinetic stabilization
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The insulin aggregation is promoted under conditions favoring partly destabilized monomers and dimers, such as low pH, high temperature, and contact with hydrophobic media (e
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Genetics, diet and lifestyle all are factors in the development of hypertension
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RESULTSThe base pool of studied cases included 39 epithelial mesotheliomas and 30 cases of reactive mesothelial proliferation, and all of these were tested with EGFR
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Guidelines for ATC Classification and DDD Assignment
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001 NSAIDs 95%CIEffect of Type 1 RPCumulative effect over all months -$10,900,000-$4,098,872Cumulative effect per year -$1,505,318-$565,362Effect of Type 2 RPCumulative effect over all months -$24,800,000 -$20,600,000Cumulative effect per year -$4,378,332-$3,636,312Table 5: Effect of RP on Patient NSAID ExpendituresNSAIDs Estimate p-ValueEffect of Type 1 RPCumulative effect over all months $666,340
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Some veterinary hospitals have valid relationships with state-licensed Internet pharmacies known as outsourced prescription management services
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1 percent had abused Ritalin, and 5 percent said they had abused the powerful pain reliever OxyContin
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"The outlook for Wafer at his February 2000 hearing seemed especially bleak in light of what had happened to the four Tulia defendants who had already been tried
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"The framework we call DTC advertising may inadvertently minimize the importance and power of medicines and their risks," he said
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The probability of winning a particular hand in the present simulation of blackjack was 47%, which is comparable to figures reported by Bond (1974) for casino blackjack
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