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Role of osmolytes as chemical chaperones during the refolding of aminoacylase
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Secondary structure of holo-enzyme and apoenzyme of aminoacylase using CD and FTIR spectroscopy
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This paradigm forms the basis for the use of tri- and pentapeptides, coil libraries (125,128), and fragments excised from structural databases for modeling properties such as solvent-accessible surface areas (129) in the highly denatured state
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Thermal denaturations following the ANS fluorescence did not show a native baseline, and a sigmoidal-like decreasing of the fluorescence was observed as the temperature was raised (data not shown)
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Structure and dynamics of membrane proteins as studied by infrared spectroscopy
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With respect to the fluorescence emission spectra α-amylases in NDSB solutions show peak maxima for the folded state which are significantly blue shifted as compared to pure buffer samples
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Azodicarboxylic acid (diamide) was used to oxidize the cysteines to form the disulfide bridge and was present in all NMR samples
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Backbone dynamics and energetics of a calmodulin domain mutant exchanging between closed and open conformations
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20 mg/ml unfolded TTR at pH 2
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DCVJ has not to our knowledge been used before on an amyloid system but has been employed on microtubule polymerization (Haidekker et al
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This agrees with the trend observed by CD and light scattering; for example, the heating curves recorded at 11 K/h show that apoA-1:DMPC disks undergo protein unfolding and lipoprotein fusion at higher temperatures than similar disks containing apoA-2 (Fig
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Thermal behavior of human plasma high density lipoprotein
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Furthermore, vertical bars in P
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Malin became interested in a cometary globule called CG4 when photographs taken with the United Kingdom Schmidt Telescope in Epping showed that the object appeared dramatically different depending on whether it was viewed through a red or a blue filter
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Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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This study suggests that the use of a defined medium containing TGF-[beta] is necessary to induce the re-expression of a differentiated chondrocytic phenotype and the subsequent stimulation of glycosaminoglycan and type-II collagen production by human monolayer expanded chondrocytes
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DiscussionThis study tests the hypothesis that a defined culture medium containing TGF-[beta]1 enhances the re-expression of a chondrocytic phenotype and the subsequent production of cartilaginous extracellular matrix by human chondrocytes used in ACI
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Health Care?" Health Affairs 22 (3): 16-30
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Commission on Presidential Debates and a board member of the International Republican Institute, which he founded in 1984
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5 times the number in 2002 and 56 percent of totalarrivals, up from 37 percent in 2002, according to government data
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