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In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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In one series of DSC experiments, the heat capacity C^sub p^(T) was recorded from the same sample during repetitive heating from 5 to 100°C followed by incubation at 24°C for 0-48 h (Fig
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RESULTS AND DISCUSSIONPrefibrillar stageFig
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3-nm in diameter, with elongated features (Khurana et al
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Manuscript approved for publication 16 September 2004 by the Scientific Editor
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