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. Am J Respir Crit Care Med 2002; 166:485-489(50) Hodge S, Hodge G, Scicchitano R, et al
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. Here, the much greater intensity enhancement observed for the mutants, compared to the WT, stands to indicate that the number of ANS binding sites and, consequently, the magnitude of the structural alteration are sensibly larger with these less stable azurins
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. This is in contrast with stabilization by kosmotropic buffers, which decrease the free energy of the native state
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. With increasing mass fraction, the expected increase in η^sub r,0^ with φ is less than expected
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. 9) diverges provide an indication of the position of the liquid-glass transition
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. Prescription drugs: their use and abuseTEACHER'S EDITIONPrescription Drug Abuse: Teens in Danger--A message from Dr
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. The purity of stock GR preparations was routinely verified by SDS-PAGE, followed by Coomassie-Blue staining
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. 8 B shows that it is in fact impossible to find room for small or large cavities unless the cavity is located sufficiently far from the center of mass of the folded protein
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. The interfacial layer can form both at an air/water (54-57) and an immiscible liquid/ water interface (58,59)
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. Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0
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. DCVJ should be especially useful for dynamic studies such as aggregation kinetics and detection of prefibrillar oligomers and can be used at acidic pH, which make this an attractive tool for in vitro studies
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. The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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. The corresponding changes in entropy are shown in Fig
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