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. Steady-state intrinsic fluorescence Protein samples were excited at 280 and 295 nm between pH 2-12
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. Structure and stability of El^sup sc^ in the presence of effectors The addition of the effectors to EI^sup sc^ did not alter substantially the secondary structure of the protein, as shown by FTIR (Table 4)
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. As a first step in this direction, we measured the thermal unfolding transition of α-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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. Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy
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. Aggregation occurs at the protein concentrations at which phase separation occurs (The observed phase behavior is consistent with literature measurements of both the second virial coefficient (39,40) and the solid-liquid coexistence line (41)
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. 1 B''), DNA coils and globules can both be recognized, and during DNA compaction the ratio between these modes changes as in a first-order phase transition
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. Pressure would be expected to stabilize the specific interactions that occur between PH-PLCδ^sub 1^ and PI(4,5)P^sub 2^ although enhancement of binding under pressure has not been observed (Rebecchi et al
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. 6, A and B), covering the main area of concentration of P
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. 3 61EH-04/9965[degrees]14'Pseudopercis1449
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. We therefore used the low molecular weight IPP polymer (PP3825, 22 µm particle size) in the subsequent tests
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.Fluorescence measurementsFluorescence measurements were carried out on an ISS PC1 (ISS Inc
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. The synchronous and asynchronous correlation plots were constructed from non normalized FSD spectra
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