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. The results are discussed in terms of the mechanism of GR folding and dimerization
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. The malaria resistance conferred by the sickle-cell gene is a pleiotropic effect
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. However, considerable structural changes were observed for ACYI when heated to 60°C (26)
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. Aminoacylase activity assay The enzyme was dissolved in 30 mM Tris-HCl, pH 7
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. Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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. Conformational substates in proteins
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. The qualitative structural implication is, therefore, that the second CaM monomer in the dimeric complex is placed along this axis in such a way that the tips of the N- and C-terminal domains touch each other
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. Chemical cross-linkingA total of 100 µl of aliquots from a TTR misfolding reaction was withdrawn al different time points, and 100 mM phosphate buffer was added in addition to 10 µl of glutaraldehyde (25%, electron microscopy grade (Sigma, St
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. All results were tit to a binding isotherm after correcting for dilution and background scattering
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. To determine whether this could be the case for PLCβ^sub 2^, we monitored the change in emission of CPM-PLCβ^sub 2^ under pressure
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. 1 B, solid circles) mainly reflects the formation of the protein-containing vesicles rather than intact-size disks
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. Specifically, the pressure-induced growth of the circular insulin amyloid suggests anisotropic packing within an ambient protofilament
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. 4, A and B), and the all-atom values are still in the buildup phase throughout the 8-ns trajectories (Fig
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. (8) Most of these children never receive proper training on gun use and safety
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. 10 This is demonstrated by the production of type-I collagen as opposed to type-II collagen which is characteristic of hyaline cartilage
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. With respect to his negative critique, we may briefly outline here three of his cases in point, namely, cancer, thalidomide and surgical training
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. Interestingly, despite the opportunity for more rational play in blackjack, Griffin (1987) and Wagenaar (1988) both reported that players tend to behave irrationally when playing this game
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. There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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. The IR difference absorption spectra were calculated by subtracting the spectra at given temperatures from the spectrum recorded at 30°C (Fig
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. © 2007 by the Biophysical Society 0006-3495/07/01/578/10 $2
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. Our approach suggests that thermodynamical analysis might reveal subtle conformational changes
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.ChEs provide a particularly suitable system for studying relationships between activity, ligand binding, unfolding, and thermal molecular fluctuations due to the following properties:1
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. This probe becomes fluorescent when it reacts with cysteine side chains (see Molecular Probes Handbook, Eugene, OR)
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. Thus the high-temperature DSC and CD transition remains invariant in the consecutive scans, yet it leads to a thermodynamically irreversible disk-to-vesicle conversion
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.5 nm observed after sample exposure to high temperatures (lanes 5 and 6 in Fig
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. The effect of apolipoprotein A-II on the structure and function of apolipoprotein A-I in a homogeneous reconstituted high density lipoprotein particle
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. Throughout all experiments, only ultrapure water (resistivity > 18 MΩ cm) was used
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.One advantage of approaches based on the covariance matrix of atomic fluctuation is the possibility to compute this quantity for different subsets of atoms or degrees of freedom
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. Since the IPP toner had a high melt flow rate at 200°C, it flowed readily; penetrated the fabric, and coated individual fibers
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. Exp Cell Res 1993:204:268-77
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., on which he had plans to build a CVS pharmacyBart Didden used to own a property in Port Chester, N
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.--Medicinal ChemistryWashington State University College of Pharmacy MHPA--Health Policy and Administration M
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