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. They are clustered in the CaM:SEF2-1mp binding interface, the CaM:CaM dimer interface, and in the flexible helix connecting the CaM N- and C-terminal domains, and have similar exchange times (~50 µs), suggesting a cooperative mechanism probably caused by protein:protein interactions
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. However, repeating this study for solubilized proteins that were freshly dialyzed to remove detergent gave an apparent K^sub d^ only 10-fold weaker than predicted, suggesting that we may be not be viewing a simple protein-protein association (K^sub d^ ~ 70 nM; Fig
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.[FIGURE 6 OMITTED]DiscussionLiterature describing the early stages of species belonging to the family Pinguipedidae (formerly Mugiloididae) is scarce
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