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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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A hypothesis for the pathway of the thermally-induced unfolding of bovine pancreatic ribonuclease
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4 using a contour plot of the two-dimensional distribution function ρ(R^sub g^/N^sup 0
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13 is the Gaussian distribution for P(x) that fits a Flory random coil
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For example, problems arise from the intrinsic temperature dependence of the tryptophan fluorescence emission and from a pronounced protein concentration dependence, which leads to a significant scattering in the data
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At physiological temperatures, atomic mean-square displacements (MSDs) measured by IENS reflect sampling of conformational substates (entropy) as well as vibrational amplitudes
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The preparation contained approximately equal amounts of BLG A (mass 18365) and BLG B (mass 18278)
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In the experiments with samples with aggregated proteins, the monomeric form was mixed with a small contribution from considerably larger particles (giving essentially a baseline), making a detailed analysis difficult
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Aggregates are expected to have rotational correlation times considerably longer than the decay time of the used dyes (e
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The ANS and DCVJ fluorescence thereafter increase over time as oligomers and protofibrils form and reach a plateau after a few hours
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9 everything he needed to know about life
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