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GR is a 108-kDa, homodimeric flavoenzyme that catalyzes the [beta]-nicotinamide-adenine dinucleotide phosphate (NADPH)-dependent reduction of oxidized glutathione (GSSG) to its reduced form (GSH), thus participating in cellular defense mechanisms against oxidative stress
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Exploring the origins of topological frustration: design of a minimally frustrated fragment of the B domain of protein A
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Eur Respir J 2005; 25:447-454(8) Vandivier RW, Fadok VA, Hoffmann PR, et al
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4 C shows the data recorded in the first and second jump from 25 to 85°C
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Linked folding and anion binding of the bacillus subtilis ribonuclease P protein
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The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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. The order of events could be characterized as the intensity decrease of the band at 1629 cm^sup -1^ > the intensity increase of the band at 1617 cm^sup -1^ > the intensity decrease of the band at 1649 and 1658 cm^sup -1^
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. In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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.How has such a remarkable reduction in folding time been achieved without the use of a spatially "funneled" energy landscape? Of course energetic interactions have been implied, but these have not been of the spatially-extended type
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. The V4 loop (residues 398-409), for which electron density is missing in all crystal structures, and the V3 loop, which has been proposed to undergo significant rearrangements upon CD4 binding, were modeled onto the gp120 core, together with the six core mutations of the LAI strain (98
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