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This suggests that the association of hydrophobic moieties might follow the disruption of secondary structures from N to A states
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The maximum number of interactions of either kind operate in the distance range of 3
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Similar data were obtained for all protein sequences shown in Table 1
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EI proteins are composed of two domains (6,7): a proteolytically inert N-terminal (EIN), and a proteolytically unstable C-terminal domain (EIC)
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1 C) between pH 7 and 8
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two peaks with different thermodynamical properties are observed, one corresponding to EIN (with pH-independent T^sub m^, values) and a second corresponding to EIC (with pH-dependent T^sub m^-values) (6)
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(36) In contrast, the phagocytosis of lysed neutrophils is much less effective at inducing TGF-[beta]1 production and inhibiting inflammatory mediator production
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This work was supported through the core strategic grant of the United Kingdom Biotechnology and Biological Sciences Research Council
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In practice, the large difference in anisotropy decay between monomeric forms lead to a broad baseline with the contribution of the smaller monomeric forms contributing as a decay curve superimposed onto a baseline
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2) and transmission electron microscopy (Fig
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Disk formation was confirmed by negative staining electron microscopy (EM) and non-denaturing gel electrophoresis which showed no free protein
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5, in which we assume that the transition state energy depends on denaturant concentration in the same way as the gutter energy, for the sake of illustration
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, 2001) was used for the MD simulations with the GROMOS96 43A1 force field (Daura et al
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12 Thus far, immunohistochemistry has played only an adjunct, minor role in differentiating mesothelioma from reactive mesothelial proliferations
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We therefore used the Newey-West autocorrelation consistent covariance matrix estimator (Newey and West 1987) throughout to ensure that hypothesis testing was valid in the presence of up to a 4 month autocorrelation
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Journal of Social Psychology, 133, 507-511
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