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Cash-strapped companies, meanwhile, often don't bother to negotiate extended payment terms
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. Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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. On freezing of the solution, however, the protein sharply enhances the probe fluorescence and shifts its spectrum to the blue
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. 1, A and B), confirm that cold denaturation is an intrinsic property of the marginally stable S-S linked apoA-2 dimer
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. Projects in development or awaiting approval include MDMA studies in Spain and Israel, a Russian study of ketamine as a treatment for heroin addicts, and a U
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. The SOGS was employed in the current study as both an indicator and quantifier of potential problem gambling among the study's participants
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. Partial motivation for this reference line comes from the work of Zagrovic and Pande (78), who showed that internal distances in unfolded ensembles of several proteins follow the predictions of the ideal random-flight chain with link length of 3
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. Protein expression and purification The EI^sup sc^ clone comprises residues 1-576, with an extra methionine and a His^sub 6^-tag at the N-terminus (15)
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. A homodimeric peptide corresponding to the complete SEF2-1 basic sequence, henceforth called SEF2-1mp, was chosen as a good model system for NMR studies of the interactions between bHLH proteins and Ca^sup 2+^/CaM
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.MATERIALS AND METHODSSample preparationSEF2-1mp mimics the DNA- and CaM-binding region of the bHLH transcription factor SEF2-1
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. The different activities in elongated DNA and compact DNA correspond to the two very different DNA molecular environments
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. 2 and show that the limiting anisotropy level is almost reached after 2 h, which is before the appearance of very large aggregates such as protofibrils and spheres, but is at the peak of the 300-500 kD oligomers
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. This reduced affinity allows both Gα(GTP) and Gβγ to bind to and change the catalytic activity of a variety of intracellular effector proteins
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. The average particle size increased with increasing dissolving temperature (step 2 in Figure 3) from a level of about 10-35 µm at 120 and 130°C to a level of about 40-65 µm at 140, 150, and 160°C
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