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The IR difference absorption spectra were calculated by subtracting the spectra at given temperatures from the spectrum recorded at 30°C (Fig
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Structural characterization of unfolded states of apomyoglobin using residual dipolar couplings
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A stressed condition of the native structure in ice appears to be common to most proteins even if oligomers seem to be more susceptible than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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The excitation light, provided by a Cermax xenon lamp (LX150UV, ILC Technology, Sunnyvale, CA), is selected (λ^sub ex^ = 350 nm for ANS and 285 nm for azurin fluorescence, respectively, 6 nm bandwidth) by a 0
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1Oa and c as higher for Sn-Ag-Cu-Bi solder (1
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The aqueous solutions were filtered through a 0
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ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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In their lipid-free state, apoA-2 and other exchangeable apolipoproteins form marginally stable molten globule with substantial helix content but nonspecific tertiary structures (Gursky and Atkinson, 1996a,b)
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Instituto de Biologia Marina y Pesquera "Alte
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One machining process involving aluminum wheel knuckles provides an example
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All ANCOVA analyses were conducted by using multiple linear regression with the function lm in S-PLUS 2000 (MathSoft, Inc
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Extended and docked conformations of the hairpin ribozyme were also resolved and quantified in previous ensemble trFRET experiments (N
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J Bone Joint Surg [Br] 1967:49-6:211-28
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