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. This suggested that the thermal unfolding of ACYI might involve step-wise transitions and the change of the hand at 1628 cm^sup -1^ preceded those of the other hands
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. Measurements of PLCβ-Gβγ associations under pressurePLCβ-Gβγ associations were monitored as a function of pressure by either following the changes in fluorescence of CPM-PLCβ bound to Gβγ or by the changes in fluorescence of CPM-Gβγ bound to PLCβ
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. However, as indicated by the z-test, the magnitude of the correlation coefficients between the SLSI and the MCS-12 did not vary significantly across the three respective class years
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. CPM-PLCβ^sub 2^ emission is responsive to the binding of Gβγ, which occurs in the nanomolar concentration range, and is also responsive to the activating peptide Gβ86-105, which occurs in the micromolar concentration range
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. Using the hairpin ribozyme as a model system, we specifically address a key question in RNA folding-how do preformed secondary structure elements dictate the course of subsequent tertiary folding events? Currently, little is known about how secondary structural elements affect the ribozyme conformational distributions important in the course of folding
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