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. Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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. It was difficult to distinguish in this research whether the pretransitional changes occurred in the catalytic domain or the dimerization domain of ACYI
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. The dialyzed solution was centrifuged at 8000 rpm for 15 min and the clear supernatant was loaded on a lactosylamine Biogel P-150 column preequilibrated wilh buffer (21)
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. As the EIN^sup st^ and EIN^sup ec^ have pH-indepcndent T^sub m^ values close to 328 K (4,5), and thus similar to the T^sub m^ value measured in EI^sup sc^ (Table 2)
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