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. Unfolding was monitored by a variety of techniques, including intrinsic fluorescence emission, anisotropy and iodide quenching measurements, far-ultraviolet circular dichroism and thiol reactivity measurements
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. The preceding discussion suggests that harshly denaturing (as opposed to mildly denaturing) conditions can be thought of as close mimics of "perfect", rather than just good, solvents (50)
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. Fluorescence experiments at pH 7 and 9 showed a sigmoidal transition with T^sub m^ = 311 ± 4 K (Fig
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