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. The cooperativity coefficients (n) are all close to unity: 1
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. Assuming an average lifetime of 3 ns for Trp in aqueous solution (Ferreira, 1989), the resulting value of K^sub SV^ for iodide quenching of Trp is 19
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. American Institute of Physics, New York
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.01 kJ K^sup -1^ mol^sup -1^ (solid line in Fig
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.In the first application of diffusion spFRET to study RNA folding, we have investigated the magnesium-induced tertiary folding of the hairpin ribozyme
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. The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig
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