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. Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism
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. The latter refers to conditions under which the conformational ensemble can be modeled by ignoring all interactions except "two-body" repulsive (steric) interactions of the excluded volume (EV) kind
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. Since, EI^sup SC^ shows a sigmoidal thermal transition at neutral pH
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. Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding
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. If R is the ANS/azurin molar ratio near or above the saturation point and M the corresponding fluorescence enhancement factor, M = F^sub max^/F^sub control^, then n = 0
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. Direct determination of vibrational density of states change on ligand binding to a protein
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. 12 ml g^sup -1^
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. Thus, pressure methods allow for the estimation in the volume associated with the Gα^sub s^(GDP) deactivation of the PLCβ-Gβγ complex even though the change in interaction energy between PLCb and Gβγ caused by Gα^sub s^(GDP) could not be detected by spectroscopic atmospheric pressure measurements
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. Mature human plasma HDL and LDL are also stabilized by kinetic barriers, suggesting that such barriers provide a universal natural strategy for lipoprotein stabilization (Mehta et al
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. Only completely dry samples were subjected to AFM-imaging
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. 4-nm solute-wall minimum distance
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. 4 NA oil immersion objective (Zeiss)
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. A 96 well plate was used and 40 µl of both the standards and samples were pipetted into each well; 250 µl of DMB solution was added to each and the absorbance at 595 nm was determined using a microplate reader (Bio-Rad Ltd, Hemel Hempstead, UK)
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. Twenty-three of the 123 participants who indicated they hadgambled in the previous 6 months did not denote a specific game-typepreference
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