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Representative isothermal and thermal denaturation profiles show that glycosylation accounts for a stabilization of ~9 kcal/mol of the tetramer, whereas the difference in T^sub m^ between the two forms is 26°C
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At lower pHs an increase in ionic strength screens the electrostatic repulsion of positive charges on the monomer and stabilizes the dimer (25,26)
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1 as measured by Grönwall (41) is plotted in Fig
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5 A, we present results showing that the affinity between CPM-PLCβ and Gβγ at atmospheric pressure on lipid membranes is strong and matches the K^sub d^(app) (i
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Thus, the fast unfolding phase is observed in disks and in the mixture of lipid-poor and vesicle-bound apoA-2, whereas the slow unfolding phase is observed in disks only
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