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. The dialyzed solution was centrifuged at 8000 rpm for 15 min and the clear supernatant was loaded on a lactosylamine Biogel P-150 column preequilibrated wilh buffer (21)
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. Even after increasing the metal ion concentration to as high as 30 mM, the refolding yields remain unchanged
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. For example, the stability of the porcine odorant hinding protein is 4
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. Direct characterization of the folded, unfolded, and urea-denatured states of the C-terminal domain of the ribosomal protein L9
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. We observed that at physiological pH as the concentration of protein was increased, the value of K^sub sv^ was reduced: 1
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. Edificio Torregaitán, Universidad Miguel Hernández, Avda
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. Taken together, these data indicate that heating of the apoA-2:DMPC disks to 99°C leads to protein unfolding and disk-to-vesicle fusion; consecutive cooling leads to a gradual reduction in the vesicle size and partial protein refolding, followed by formation of smaller protein-containing vesicles and additional helical refolding at 34-24°C
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. These intermolecular contacts restrict the mobility of the involved residues and, in turn, their configurational entropy
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. THERMAL ANALYSISWe determined the crystalline melting temperatures T^sub m^, of the IPP, EVA 9%, and EVA 19% polymers by DSC as 165, 83, and 97°C, respectively
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. Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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. Usually, glycans do not generate permanent secondary structure but trigger local eonformational alterations close to the glycosylation site, particularly by inducing a β-turn at the site (13)
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. DATA ANALYSIS DISCUSSION It is reasonably clear that the presence of glycosylation does affect the expression of the protein into its final mature form
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. (33) The second pathway includes ced-1, ced-6, and ced-7 (representing the mammalian homologues LRP, GULP, and ABC-A1 or ABC-A7, respectively)
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.How Aggregation and Conformational Scrambling of Unfolded States Govern Fluorescence Emission SpectraDuy, CABSTRACT In a case study on five homologous α-amylases we analyzed the properties of unfolded states as obtained from treatments with GndHCl and with elevated temperatures
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. For parameter values of τ^sub is^ = 2
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