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The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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(13,16,38) The suppressive effect of efferocytosis on the adaptive immune system appears to be largely PS-dependent, to include both TGF-[beta]1-dependent and TGF-[beta] 1-independent mechanisms, and to result in decreased antigen-specific CD4 T cells, antigen-specific IgG, dendritic cell maturation, and IL-12 production
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from LaRoche, Mannheim, Germany) was obtained in ammonium sulfate solution
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This loss is compensated via multimerization
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Early measurements (42) showed BLG has a dipole moment of a sufficient magnitude to generate significant attractive dipole-dipole interactions which are predicted to be screened by increasing ionic strength at the concentrations relevant to this study (40,43)
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Hard-sphere statistics along the metastable amorphous branch
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Sample solutionsSolutions for microscopic observations were prepared by mixing PEG 3000 or PEG 10000 in twice-distilled water until dissolution, adding T4 DNA with further mixing and incubation for 30 min, adding DAPI with further mixing and incubation for 30 min, and finally adding alkali chloride solution and letting the sample solution sit for 1-2 h before observations
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Protein association to membranes is stabilized by a combination of electrostatics as well as polar and hydrocarbon interactions
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© 2005 by the Biophysical Society0006-3495/05/02/1344/10 $2
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Comparacion del esqueleto oseo de dos especies de peees de la familia Pinguipedidae
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This truncated form of gp120 (core gp120) does not compromise much the biophysical and biological properties compared to the fulllength wild-type (Kwong et al
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None of the eggs fixed just after collection were morphologically identifiable
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Sections were rehydrated and incubated in 200 µL hydrogen peroxide in 12 ml methanol to block the activity of endogenous peroxidase and then pre-treated in 0
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