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Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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of Biomedical Engineering and Center for Computational Biology, Washington University in St
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For this purpose the same experimental setup (temperature controlling unit, quartz-cuvettes with 1 cm optical pathlength) was used as for fluorescence studies
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These data reveal an increased flexibility of native HuBChE relative to its soman-inhibited counterpart above 334 K (61°C)
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Heteronuclear (H-1,C-13,N-15) NMR assignments and secondary structure of the basic rcgion-helix-loop-helix domain of E47
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As the associated shear stress was [much less than]50 kPa, we ascribe this behavior to being equivalent to the η^sub 0^ of hard sphere colloidal suspensions
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Na^sup +^ Shows a Markedly Higher Potential than K^sup +^ in DNA Compaction in a Crowded EnvironmentZinchenko, Anatoly AABSTRACTWhereas many physicochemical investigations have shown that among monovalent cations Na+ ion possesses minimal potential for DNA binding, biological assays have shown that Na+ ion (in contrast to K+ ion) plays a primary role in chromatin compaction and related processes
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Eocating monovalent cations in the grooves of B-DNA
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Its yolk sac was small and the single oil globule was located on the anterior part of the yolk mass
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When comparing the melting rates of the three polymers, the melting temperatures should be considered
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20Cells not required for the ACI procedures were then assessed for viability within an hour of the operative procedure
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Differences between the values obtained for each culture medium were analysed using the unpaired Student's t-test
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Values of p ResultsCell viability
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. In essence, the structure consisted of an α-helix subdomain, which is a four-helix bundle, comprising α-helices 1-4; an α/β subdomain, comprising a β-sandwich, formed by a four-stranded parallel β-sheet (β-strands 1-4) and a three-stranded antiparallel β-sheet (β-strands 1, 5, and 6), and three short helices (α-helices 5-7) that contain the phosphorylation site (His^sup 186^) (Fig
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. AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR
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. 1 shows the change in ANS fluorescence induced by the four azurin species upon freezing of the sample at -13°C (200 µM ANS, 2
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. Aggregation of acid unfolded TTR (23°C, 14 µM monomer concentration) was induced by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM
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. Second, the heat-unfolding curve of the apoA-2:DMPC disks is sigmoidal and is shifted to higher temperatures as compared to that of free protein; thus the unfolding of free apoA-2 is complete at 80°C, well below the apparent melting temperature T^sub m^ = 84°C of the apoA-2:DMPC complexes observed at this scan rate (Fig
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. Similarly, repetitive T-jumps with DMPC disks containing apoA-1 and apoC-1 show faster unfolding in the second and consecutive jumps (data not shown)
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