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. Further, the unfolding pathways in the two are widely different
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. The former corresponds to that of the intermediate, whereas the latter peak represents the partially denatured population
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. (34)[FIGURE 3 OMITTED]Efferocytosis also increases the production of secretory leukoprotease inhibitor, which is an antiprotease with activity against elastase and cathepsin G
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. Elastase-mediated phosphatidylserine receptor cleavage impairs apoptotic cell clearance in cystic fibrosis and bronchiectasis
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. 2-cm cell at a protein concentration of 0
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. Intracellular molecules have smaller diffusion coefficients than extracellular molecules because intracellular and compartmental boundaries restrict diffusion
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. In this case, a higher soldering temperature was responsible for the rapid chemical reaction that enhanced the atomic diffusion from the base metal into the solder, resulting in an increase in size of the IMCs in the bulk of the solders
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. DCVJ is intrinsically quenched and belongs to a class of fluorescent probes known as molecular rotors
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. These results gave a volume change of 34 ± 5 ml/mol
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. A 15-mL aliquot of the resulting solution was transferred into each of several 20 mL, poly(tetrafluoroethylene)-lined hydrothermal pressure vessels
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. Because this peak does not provide relevant FRET (distance) information, only the region above E = 0
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. Although subcellular variations could not be determined with this whole-cell technique, it yielded spectra with increased resolution and sensitivity, since dipolar broadening and susceptibility variation within the sample are being reduced (30)
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. From the distribution of (τ^sup o^^sub m^) values, the shape of the diffusion tensor can be determined (30), whereas the degree of anisotropy can be gauged from the maximum and minimum (τ^sup o^^sub m^)^sub app^ values
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. In the solutions containing 100 mM NaCl and 10 mM acetate buffer alone, this interaction becomes increasingly attractive at short range
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.Measurements of PLCβ-Gβγ associations under pressurePLCβ-Gβγ associations were monitored as a function of pressure by either following the changes in fluorescence of CPM-PLCβ bound to Gβγ or by the changes in fluorescence of CPM-Gβγ bound to PLCβ
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