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. The appearance of the unusual exothermic peak and the lack of endothermic peak suggested that the unfolding of ACYI was accompanied by serious aggregation, which might be a nucleation-dependent process (36)
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. The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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. The secondary structure of EI^sup sc^ upon binding was not largely affected (Table 4), but there were subtle changes in the stability of the protein (Table 3)
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. COPDApoptotic cells are increased in the lungs of COPD patients (3,8) and in animal models of COPD
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. The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II) dependent oxygenase activity
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. However, with respect to the heating rate dependence of the respective λ^sub max^-values obtained for unfolded states, TAKA exhibits the same behavior as compared to the other α-amylases (Fig
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. According to an established model of Ψ-condensation, thermodynamically unfavorable contact between DNA and PEG decreases the available free space for coil DNA in solution and DNA undergoes a collapse transition at some critical concentration of PEG
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. However, more important conclusions arise when our results are compared with previous reports on DNA binding with monocations
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. This ranking suggests that the disk stability correlates with the protein size rather than hydrophobicity
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. Evidence for sequential barriers and obligatory intermediates in apparent two-state protein folding
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. More recently, single-molecule FRET detection (Ha et al
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