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. 5 M GdnHCl), reflecting exposure of tryptophan residues to the aqueous medium (Fig
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. To the best of our knowledge these are the first studies of this kind carried out in any member of the EI family
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. In conclusion, residues with fast exchange can be identified from the combination of R^sub ex^ and ΔR^sub ex^ using either the fast exchange equations or the general fast/slow exchange equations derived by Ishima and Torchia (34)
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. Thus, the N-H^sup N^ bond vectors within an α-helix must have similar (τ^sup o^^sub m^)^sub app^, and the average (τ^sup o^^sub m^)^sub app^ for each helix can be taken to reduce the error
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. The rate determining step is not the rapid formation of local secondary structure, nor the diffusion of subdomains per se, but the organization of large pieces of secondary structure into the same topological configuration as the native state, which thereafter is able to form rapidly
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. Appendix F in Status of the Pacific coast groundfish fishery through 1994 and recommended acceptable biological catches for 1995
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. The folding of RNA is generally coupled to its biological function, and this is illustrated well by the hairpin ribozyme, a small catalytic RNA with both endonuclease and ligase activities
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. 1 is displayed in subsequent histograms
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. Fourier self-deconvolution (FSD) was performed using the software Spectrum v3
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. Either way, the thinking is that it ought to he difficult to locate spherical cavities of different sizes within protein interiors
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. This shoulder seems to represent a subpopulation of thermal unfolded states which exhibit less buried tryptophan residues, possibly related to unfolded proteins which are not aggregated
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. laevis embryos are ~1 mm in diameter, oocytes up to the 8-cell or 16-cell stages would, under optimal conditions, be observable at single-cell resolution with our (180 µm)^sup 3^ voxel size
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